Z702366
Sigma
Handbook of Molecular and Cellular Methods in Biology and Medicine, 2nd ed.
| ISBN-10: | 0-84930815-1 |
| ISBN-13: | 978-0-84930815-4 |
Description
| General description | With step-by-step protocols, this edition presents up-to-date molecular and cellular methods and techniques, including additional methods for analysis of DNA-protein interactions by DNA footprinting and gel retardation assay, mRNA differential display, and microarray technology; Explains the principles behind each method; Includes helpful trouble-shooting guides. This edition includes: new protocols in all chapters, including alternative protocols; In vitro transcription methods; analysis of DNA sequences; new bioseparation techniques. New chapters cover: mRNA differential display; Inhibition of gene expression; In situ hybridization; Combinatorial techniques; Computational data mining methods applied to combinatorial chemistry libraries. |
Properties
| publication info | L. Cseke, et al., ed., CRC Press, 2004, 600 pp., comb bound |
Table Of Contents
| Table of Contents | 1. Isolation and Purification of DNA 2. Isolation and Purification of RNA 3. Extraction and Purification of Proteins 4. Preparation of Nucleic Acid Probes 5. Southern Blot Hybridization 6. Northern Blot Hybridization 7. Preparation of Monoclonal and Polyclonal Antibodies against Specific Protein(s) 8. Western Blot Hybridization 9. cDNA Libraries 10. Genomic DNA Libraries 11. DNA Sequencing Analysis 12. DNA Mutagenesis 13. DNA Footprinting and Gel Retardation Assay 14. Differential Display 15. Functional Genomics and DNA Microarray Technology 16. Translation of mRNA(s) and Analysis of Proteins by Gel Electrophoresis 17. Plant Tissue and Cell Culture 18. Gene Transfer and Expression in Animals 19. Gene Transfer and Expression in Plants 20. Inhibition of Gene Expression 21. Light, Scanning Electron, Environmental Scanning Electron, Transmission Electron, and Confocal Microscopy 22. Localization of Gene Expression 23. Bioseparation Techniques and Their Applications 24. Combinatorial Techniques 25. Computational Data Mining Methods Applied to Combinatorial Chemistry Libraries Index |
